Burlington is a city in, and the county seat of, Des Moines County, Iowa, United States. The population was 23,982 in the 2020 census, a decline from the 26,839 population in 2000. Burlington is the center of a micropolitan area, which includes West Burlington and Middletown, Iowa, and Gulfport, Illinois. Burlington is the home of Snake Alley, the crookedest street in the world.

Des Moines County is located in the U.S. state of Iowa. As of the 2020 census, the population was 38,910. The county seat and largest city is Burlington. It is one of Iowa's two original counties along with Dubuque County; both were organized by the Michigan Territorial legislature in 1834.