State Route 140 is a state highway in the U.S. state of California, 102 miles (164 km) in length. It begins in the San Joaquin Valley at Interstate 5 near Gustine, and runs east into Sierra Nevada, terminating in Yosemite National Park.

Gustine is a city in Merced County, California. Gustine is located 29 miles (47 km) west of Merced, at an elevation of 98 feet. At the 2020 census, the city population was 6,110, up from 5,520 at the 2010 census.