Chambers's Encyclopaedia was founded in 1859 by William and Robert Chambers of Edinburgh and became one of the most important English language encyclopaedias of the 19th and 20th centuries, developing a reputation for accuracy and scholarliness that was reflected in other works produced by the Chambers publishing company. The encyclopaedia is no longer produced. A selection of illustrations and woodblocks used to produce the first two editions of the encyclopaedia can be seen on a digital resource hosted on the National Museums Scotland website.

William Chambers of Glenormiston was a Scottish publisher and politician, the brother of Robert Chambers. The brothers were influential in the mid-19th century, in both scientific and political circles.