Cherokee is a town in west Colbert County, Alabama, United States. Located near the Tennessee River, it is part of the Florence–Muscle Shoals metropolitan area, known as "The Shoals". As of the 2010 census, the population of the town was 1,048.

Colbert County is a county located in the northwestern part of the U.S. state of Alabama. As of the 2020 census the county's population was 57,227. The county seat is Tuscumbia. The largest city is Muscle Shoals.