The Chesapeake Bay is the largest estuary in the United States. The Bay is located in the Mid-Atlantic region and is primarily separated from the Atlantic Ocean by the Delmarva Peninsula, including parts of the Eastern Shore of Maryland, the Eastern Shore of Virginia, and the state of Delaware. The mouth of the Bay at its southern point is located between Cape Henry and Cape Charles. With its northern portion in Maryland and the southern part in Virginia, the Chesapeake Bay is a very important feature for the ecology and economy of those two states, as well as others surrounding within its watershed. More than 150 major rivers and streams flow into the Bay's 64,299-square-mile (166,534 km2) drainage basin, which covers parts of six states and all of Washington, D.C.

The Eastern Shore of Virginia is the easternmost region of the Commonwealth of Virginia in the United States. It consists of two counties on the Atlantic coast. It is detached from the mainland of Virginia by the Chesapeake Bay. The 70-mile-long (110 km) region is part of the Delmarva Peninsula. Its population was 45,695 as of 2020.