The Chesapeake Bay is the largest estuary in the United States. The Bay is located in the Mid-Atlantic region and is primarily separated from the Atlantic Ocean by the Delmarva Peninsula, including parts of the Eastern Shore of Maryland, the Eastern Shore of Virginia, and the state of Delaware. The mouth of the Bay at its southern point is located between Cape Henry and Cape Charles. With its northern portion in Maryland and the southern part in Virginia, the Chesapeake Bay is a very important feature for the ecology and economy of those two states, as well as others surrounding within its watershed. More than 150 major rivers and streams flow into the Bay's 64,299-square-mile (166,534 km2) drainage basin, which covers parts of six states and all of Washington, D.C.

An estuary is a partially enclosed coastal body of brackish water with one or more rivers or streams flowing into it, and with a free connection to the open sea. Estuaries form a transition zone between river environments and maritime environments and are an example of an ecotone. Estuaries are subject both to marine influences such as tides, waves, and the influx of saline water, and to fluvial influences such as flows of freshwater and sediment. The mixing of seawater and freshwater provides high levels of nutrients both in the water column and in sediment, making estuaries among the most productive natural habitats in the world.