Chiefland is a city in Levy County, Florida, United States. The population was 2,316 at the 2020 census. Chiefland calls itself "The Gem of the Suwannee Valley" and was incorporated in 1913.

Levy County is a county located on the Gulf coast in the northwestern part of the U.S. state of Florida. As of the 2020 census, the population was 42,915. Its county seat is Bronson.