Maximo Tomás Modesti is an Argentine sport shooter. He won a silver medal in the men's air pistol at the 2003 Pan American Games in Santo Domingo, Dominican Republic, was selected to represent Argentina, as the oldest member of the team, at the 2004 Summer Olympics. Having started shooting for more than three decades, Modesti trained throughout his career for the Rio Shooting Federal Club under head coach Juan Carlos Sampayo. Modesti qualified for the Argentine squad in pistol shooting at the 2004 Summer Olympics in Athens, by having achieved a mandatory Olympic standard of 576 and picking up the silver medal in the air pistol from the Pan American Games. Modesti fired 559 points to obtain the forty-fourth spot in the men's 10 m air pistol, marked a score of 548 to share a thirtieth-place finish with Chinese Taipei's Chang Yi-ning in the men's 50 m pistol, falling short of his chance to advance further into the final for both events. ISSF Profile
Immunocytochemistry is a common laboratory technique, used to anatomically visualize the localization of a specific protein or antigen in cells by use of a specific primary antibody that binds to it. The primary antibody allows visualization of the protein under a fluorescence microscope when it is bound by a secondary antibody that has a conjugated fluorophore. ICC allows researchers to evaluate whether or not cells in a particular sample express the antigen in question. In cases where an immunopositive signal is found, ICC allows researchers to determine which sub-cellular compartments are expressing the antigen. Immunocytochemistry differs from immunohistochemistry in that the former is performed on samples of intact cells that have had most, if not all, of their surrounding extracellular matrix removed; this includes individual cells that have been isolated from a block of solid tissue, cells grown within a culture, cells deposited from suspension, or cells taken from a smear. In contrast, immunohistochemical samples are sections of biological tissue, where each cell is surrounded by tissue architecture and other cells found in the intact tissue.
Immunocytochemistry is a technique used to assess the presence of a specific protein or antigen in cells by use of a specific antibody, which binds to it, thereby allowing visualization and examination under a microscope. It is a valuable tool for the determination of cellular contents from individual cells. Samples that can be analyzed include blood smears, swabs, cultured cells, cell suspensions. There are many ways to prepare cell samples for immunocytochemical analysis; each method has its own strengths and unique characteristics so the right method can be chosen for the desired sample and outcome. Cells to be stained can be attached to a solid support to allow easy handling in subsequent procedures; this can be achieved by several methods: adherent cells may be grown on microscope slides, coverslips, or an optically suitable plastic support. Suspension cells can be centrifuged onto glass slides, bound to solid support using chemical linkers, or in some cases handled in suspension. Concentrated cellular suspensions that exist in a low-viscosity medium make good candidates for smear preparations.
Dilute cell suspensions existing in a dilute medium are best suited for the preparation of cytospins through cytocentrifugation. Cell suspensions that exist in a high-viscosity medium, are best suited to be tested as swab preparations; the constant among these preparations is. For any intercellular reaction to take place, immunoglobulin must first traverse the cell membrane, intact in these preparations. Reactions taking place in the nucleus can be more difficult, the extracellular fluids can create unique obstacles in the performance of immunocytochemistry. In this situation, permeabilizing cells using detergent or choosing organic fixatives becomes necessary. Antibodies are an important tool for demonstrating both the presence and the subcellular localization of an antigen. Cell staining is a versatile technique and, if the antigen is localized, can detect as few as a thousand antigen molecules in a cell. In some circumstances, cell staining may be used to determine the approximate concentration of an antigen by an image analyzer.
There are many methods to obtain immunological detection on tissues, including those tied directly to primary antibodies or antisera. A direct method involves the use of a detectable tag directly to the antibody, allowed to bind to the antigen in a cell. Alternatively, there are many indirect methods. In one such method, the antigen is bound by a primary antibody, amplified by use of a secondary antibody which binds to the primary antibody. Next, a tertiary reagent containing an enzymatic moiety is applied and binds to the secondary antibody; when the quaternary reagent, or substrate, is applied, the enzymatic end of the tertiary reagent converts the substrate into a pigment reaction product, which produces a color in the same location that the original primary antibody recognized that antigen of interest. Some examples of substrates used are AEC, or DAB. Use of one of these reagents after exposure to the necessary enzyme produces a positive immunoreaction product. Immunocytochemical visualization of specific antigens of interest can be used when a less specific stain like H&E cannot be used for a diagnosis to be made or to provide additional predictive information regarding treatment.
Alternatively the secondary antibody may be covalently linked to a fluorophore, detected in a fluorescence or confocal microscope. The location of fluorescence will vary according to the target molecule, external for membrane proteins, internal for cytoplasmic proteins. In this way immunofluorescence is a powerful technique when combined with confocal microscopy for studying the location of proteins and dynamic processes. Immunocytochemistry Staining Protocol Immunohistochemistry of Whole-Mount Mouse Embryos
The Broken Tower is a 2011 American biographical drama film directed, produced, edited by and starring James Franco. The film was made by Franco as his Masters thesis for his MFA in filmmaking from New York University; the film is about American poet Hart Crane. Franco appears in the starring role as Crane along with Michael Shannon as one of Crane's lovers; the Broken Tower made its world premiere in April 2011 at Boston College. It was shown at the 2011 Los Angeles Film Festival in June and released on DVD in 2012; the film includes the recitation by Franco of several of Crane's poems both as voice-over additions to the film, as well as actual readings of several poems rendered by Franco as portraying Crane himself. The film begins with a plot structure based on a progression of chapters titled as "Voyages" in Hart Crane's life loosely related to Crane's lyric poem of the same name. In the first "Voyages", a depiction is made of an early attempt by Crane to take his own life. Among the other opening "Voyages", the audience is shown depictions of several same sex relationships which Crane had throughout his lifetime in semi-graphic portrayal consistent with the film's rating.
Crane's life is shown progressing through the various "Voyages" in the film portrayed through his troubled relationship with the father, his close relationship to his mother, his frustrating relationship to his job in advertising as a copyrighter in New York City. In the final "Voyages," Crane's difficult relationship to alcoholism is depicted, ending with his final "Voyage" on a small cruise ship at sea in the vicinity of Mexico where Crane ended his life by his own hand. James Franco – Hart Crane Michael Shannon – Emile Opffer Dave Franco – Young Hart Richard Abate – Father Crane Betsy Franco – Mother Crane Paul Mariani – Alfred Stieglitz Shandor Garrison – Gorham Munson Stacey Miller – Mrs. Cowley Franco thought of the idea for the film while reading Paul Mariani’s biography of Crane entitled The Broken Tower after the name of one of Crane's poems, on the set of his 2002 movie Sonny. Franco had publicly stated; the DVD release of the film includes a supplement. The film's world premiere was held at Boston College on April 15, 2011.
Franco chose to debut it at that venue there. The Broken Tower was screened at the 2011 Los Angeles Film Festival on June 20, it was among more than 200 feature films, short projects, music videos from more than 30 countries to be selected. It was released on DVD March 2012. Critical response to the film was negative. In one critical review, Elizabeth Weitzman from the New York Daily News writes, "It’s not an insult to say the black-and-white film looks like a grad-school thesis project, since that’s what it is... But that does mean you should be prepared for some high-minded pretension, lots of self-consciously arty shots, long stretches of profound nothingness." Although Weitzman is critical of Franco's writing and directing, she does compliment the film's cinematography and Franco's acting. In a review in The Village Voice, Melissa Anderson wrote that the film was "sincere and misguided" and that it was full of literary biopic cliches; the Broken Tower 2011 in film The Broken Tower on IMDb
Values And Virtues is an EP by former Dum Dums vocalist Josh Doyle. "High School Soldier" - 3:50 "Ghosts Like You" - 4:18 "Pop Idol" - 2:55 "Waiting For The Payoff" - 4:46 "Concrete Moon" - 4:03 "Middletown" - 3:24 "Two Lines Instead Of One" - 3:36 "This Transcendant Ache" - 3:25 "My Jerusalem" - 3:53 "Waiting For The Payoff" - 4:18 "Jericho" - 3:24 "Damaged Goods" - 4:17 "Army Of Two" - 4:08 "Concrete Moon" - 4:56 Written by Josh Doyle Track 2, 4 & 5 produced by Justin Saunders Track 1 & 3 produced and mixed by Joe Baldridge Track 2, 4 & 5 mixed by John Castelli Mastered by Steve Wilson Artwork & design by Shaun Gordon Vocals & guitar by Josh Doyle Additional guitars by Mark Hamilton, Justin Saunders & Jason Moore Bass by Jordan Hester, Beau Burtnick, Tony Lucido & Josh Fink Drums by Paul Evans, Doy Gardner & Joshua Moore Backing vocals by Jason Moore Cello by Justin Saunders
Mount Diwata locally unofficially nicknamed Diwalwal, is a remote 1,261-metre high range of volcanic mountain and biodiversity area in Davao Region on eastern part of Mindanao island of Philippines. Rich in gold and copper ores and mines, it is spread across the 3 municipalities: Monkayo in the province of Compostela Valley. Laguna Copperplate Inscription, the oldest written document in Philippines dated to 900 CE, refers to Mount Diwata, it is host to multiple high–grade epithermal gold vein systems and porphyry copper deposits. Mount Diwata is now a barangay of Monkayo, with an estimated population of 12,000 to 20,000 in 2015 in shanty towns of miners and their families including 1,000 female miners, it is located 160 km from Davao Airport, 80 km from Tagum City, 135 km from Hinatuan, Surigao del Sur, 185 km from Butuan City, 255 km from Malaybalay, 300 km from Surigao City airport and 363 km from Cagayan de Oro airport. The mountain is named after the diwata, the pre-Spanish native faith based on the Indianized concept of devata in Hinduism in Indosphere.
Its local nickname "diwalwal" is dervived from the native idiom "diwalwal ang dila" ("one's tongue hanging out"}. According to the oral tradition, a king of this area fell in love, married a poor but beautiful and brave woman named "Diwata", she fought the enemies of kingdom and she was killed fighting in one such battle while she was pregnant. The king named her mountain burial place "Diwata" after his beloved martyred wife. Over time the villagers started to perceive that the shape of mountain range resembled with a pregnant woman, the name of mountain range where she was buried came to be known as the "Mount Diwata", she as the native protective ancestor, "Diwata", is considered the enchanting guardian angel/goddess of this beautiful mountain range and ecology. The Laguna Copperplate Inscription, dated to 900 CE, which refers to Mount Diwata; the inscription is a legal document inscribed on a copper plate in 900 CE, is the earliest known written document found in the Philippines. Inscription mentions that the "Jayadewa" of Tondo is invoking the authority of the "Chief of Dewata" as P. Patanñe notes: "This relationship is unclear but a possible explanation is that the chief of Dewata wanted it to be known that he had a royal connection in Java.”
Scholars after Postma, such as Patanñe and Abinales and Amoroso have come to identify the Dewata of the inscription as a settlement in or near “present-day Mount Diwata, near Butuan”. In September 1983, gold was discovered by a Lumad in the creeks of Mount Diwata, leading to gold rush causing the population to rise to 125,000, who were miners living in shanties along the 2 km logging road where the gold mine valcanoic veins are located. On 25 November 2002, due to the unsafe and lethal illegal mining and siltation of Naboc River, the government declared 8,100 hectares area of Mount Diwalwal across Agusan-Davao-Surigao a "Mineral Reservation" via the Proclamation No. 297. Mining was taken over by the government owned corporation. Mining prospects include high grade ores in Buenas-Tinago vein systems. According to BirdLife International, Mount Diwata Important Bird Area is the forested area at the southern end of the Diwata range to the south of Mount Hilong-Hilong with highest peak rising to 1,261m and several lower slopes which habit many and restricted species of the Mindanao and Eastern Visayas Endemic Bird Area.
The threatened species of birds in Mount Diwata range are Philippine Eagle, Mindanao Bleeding-heart, Philippine Kingfisher, Blue-capped Kingfisher, Wattled Broadbill Philippine leafbird, White Lauan,Red Lauan, Yakal, Tangili. Mountain rage has numerous waterfalls of which 34 waterfalls, 4 hot springs, 14 caves, 34 lakes and one campsite "Datu Lipus Macapandong Eco-Tourism Park" in the area of the range falling in the Agusan del Sur province. One of the popular fall is "Bega fall", accessible from Agusan del Sur side."Datu Lipus Macapandong Eco-Tourism Park", a Boys Scout camping site, near the natural water pool of the waterfall which offers the nice view of various summits. There are several peaks, each of which can be ascended through several different routes, through the palm oil forests on the lower and coconut trees on the higher mountain slopes, meadows, butterfly infested rattan and Romblon and rattan forests. Governor of Agusan del Sur had built the "Stairway to Heaven" consisting of concrete stairs and resting shelters for climbers.
San Francisco Mountaineering Club in the nearby town of San Francisco organises mountaineering trips for the members. The mountain range has 20m high milti-tiered "Awao waterfalls" on Monkayo side on black canyon with a natural swimming pool, a garden and kiosks nearby, its higher tier is harder to climb but still accessible. The fall can be reached from the Monkayo town by a habal-habal and a sacending trek over the rivers and creeks. Illegal and unsafe mining practices have resulted in pollution of water, mercury poisoning, silting of Noboc river, deaths of human, destruction of flora and fauna. Thousands of miner, including children, are at risk of air and water pollution and mercury p