St. Anne's Church is a Roman Catholic church in Vilnius' Old Town, on the right bank of the Vilnia River established circa 1495–1500. It is a prominent example of both Flamboyant Gothic and Brick Gothic styles. St. Anne's is a prominent landmark in the Old Town of Vilnius that enabled the district to be included in the list of UNESCO World Heritage sites, and is one of the most interesting examples of Gothic architecture in Lithuania.

The Old Town of Vilnius, one of the largest surviving medieval old towns in Northern Europe, has an area of 3.59 square kilometres. It encompasses 74 quarters, with 70 streets and lanes numbering 1487 buildings with a total floor area of 1,497,000 square meters. It was founded by the Lithuanian Grand Duke and King of Poland Jogaila in 1387 on the Magdeburg rights the oldest part of the Lithuanian capital of Vilnius, it had been developed over the course of many centuries, and has been shaped by the city's history and a constantly changing cultural influence. It is a place where some of Europe's greatest architectural styles—gothic, renaissance, baroque and neoclassical—stand side by side and complement each other. There are many Catholic, Lutheran and Orthodox churches, residential houses, cultural and architectural monuments, museums in the Old Town.