The Church of the Holy Rude is the medieval parish church of Stirling, Scotland. It is named after the Holy Rood, a relic of the True Cross on which Jesus was crucified. The church was founded in 1129 during the reign of David I, but the earliest part of the present church dates from the 15th century. As such it is the second oldest building in Stirling after Stirling Castle, parts of which date from the later 14th century. The chancel and tower were added in the 16th century.

Stirling is a city in central Scotland, 26 miles (42 km) northeast of Glasgow and 37 miles (60 km) north-west of Edinburgh. The market town, surrounded by rich farmland, grew up connecting the royal citadel, the medieval old town with its merchants and tradesmen, the Old Bridge and the port. Located on the River Forth, Stirling is the administrative centre for the Stirling council area, and is traditionally the county town of Stirlingshire. Proverbially it is the strategically important "Gateway to the Highlands". It has been said that "Stirling, like a huge brooch clasps Highlands and Lowlands together". Similarly "he who holds Stirling, holds Scotland" is sometimes attributed to Robert the Bruce. Stirling's key position as the lowest bridging point of the River Forth before it broadens towards the Firth of Forth made it a focal point for travel north or south.