The Cisalpina Private Natural Heritage Reserve is a private natural heritage reserve in the state of Mato Grosso do Sul, Brazil. It contains an area of marshland at the head of the reservoir of the Eng Sérgio Motta Dam on the Paraná River.

A private natural heritage reserve is a type of preservation area in Brazil. The land is owned privately and may be used for research, education, eco-tourism and recreation. However, this use must be compatible with the goals of preserving the environment and maintaining biodiversity. Although the private reserves have been criticized, they are a valuable complement to publicly owned reserves.