The Coke Zero Sugar 400 is an annual NASCAR Cup Series stock car race at Daytona International Speedway. First held in 1959, the event consists of 160 laps, 400-mile (640 km), and is the second of two major stock car events held at Daytona on the Cup Series circuit, the other being the Daytona 500. From its inception in 1959 through 2019, it was traditionally held on or around the United States' Independence Day. In 1998, it became the first stock car race at Daytona to be held at night under-the-lights. In 2020, the race was moved to late August.

Coca-Cola Zero Sugar is a diet cola produced by the Coca-Cola Company.