College lacrosse is played by student-athletes at colleges and universities in the United States and Canada. In both countries, men's field lacrosse and women's lacrosse are played at both the varsity and club levels. College lacrosse in Canada is sponsored by the Canadian University Field Lacrosse Association (CUFLA) and Maritime University Field Lacrosse League (MUFLL), while in the United States, varsity men's and women's lacrosse is governed by the National Collegiate Athletic Association (NCAA), National Junior College Athletic Association (NJCAA) and National Association of Intercollegiate Athletics (NAIA). There are also university lacrosse programs in the United Kingdom sponsored by British Universities and Colleges Sport (BUCS) and programs in Japan.

Field lacrosse is a full contact outdoor sport played with two opposing teams of ten players each. The sport originated among Native Americans, and the modern rules of field lacrosse were initially codified by Canadian William George Beers in 1867. Field lacrosse is one of three major versions of lacrosse played internationally. The rules of men's lacrosse differ significantly from women's field lacrosse. The two are often considered to be different sports with a common root. An outdoor six-a-side version, lacrosse sixes, was established in 2021 and features six players per team, reduced field size, and shorter duration to be conducive for daily tournament play. Another version, indoor box lacrosse, is also played under different rules.