Colorado Bureau of Investigation (CBI), a division of Colorado Department of Public Safety, is a law enforcement agency of the state of Colorado that performs forensic and laboratory services and criminal investigations at the request of local and state law enforcement agencies and district attorneys. They investigate arson, homicides, sexual assaults, fraud, cyber, identity theft, and other crimes. Their forensic services include DNA, biology, firearm, latent print, toxicology and drug chemistry analysis. Other units in the CBI include Crime Scene Services, Crime Information Management Unit, Criminal Justice Information Systems, and Colorado’s InstaCheck Unit. The CBI is designated by the Federal Bureau of Investigation (FBI), Criminal Justice Information Services (CJIS) Division, as the CJIS Systems Agency for Colorado. It is headquartered in the Denver suburb of Lakewood at 690 Kipling Street.

The Colorado State Patrol (CSP) (originally known as the Colorado State Highway Courtesy Patrol), based in Lakewood, Colorado, is a division of the Colorado Department of Public Safety, and is one of the official state patrol agencies of Colorado, along with the Colorado Bureau of Investigation (CBI), Colorado Division of Criminal Justice (DCJ), Colorado Division of Fire Prevention & Control (DFPC), and Colorado Division of Homeland Security and Emergency Management (DHSEM). Additionally, the Executive Director's Office supports operations of the five divisions and houses the Colorado School Safety Resource Center (CSSRC) and Colorado Integrated Criminal Justice Information Systems (CJIS). The CSP primarily enforces traffic laws on interstates and state highways and guards the state capitol and the Governor of Colorado.