The colossal squid is the largest member of its family Cranchiidae, the cockatoo or glass squids, with its second largest member being Megalocranchia fisheri. It is sometimes called the Antarctic cranch squid or giant squid and is believed to be the largest squid species in terms of mass. It is the only recognized member of the genus Mesonychoteuthis and is known from only a small number of specimens. The species is confirmed to reach a mass of at least 495 kilograms (1,091 lb), though the largest specimens—known only from beaks found in sperm whale stomachs—may perhaps weigh as much as 600–700 kilograms (1,300–1,500 lb), making it the largest known invertebrate. Maximum total length has been estimated between 10 metres (33 ft) and 14 metres (46 ft) but the former estimate is more likely. The colossal squid has the largest eyes of any known creature ever to exist, with an estimated diameter of 27–30 cm (11–12 in) to 40 cm (16 in) for the largest collected specimen.

Invertebrates is an umbrella term describing animals that neither develop nor retain a vertebral column, which evolved from the notochord. It is a paraphyletic grouping including all animals excluding the chordate subphylum Vertebrata, i.e. vertebrates. Well-known phyla of invertebrates include arthropods, mollusks, annelids, echinoderms, flatworms, cnidarians and sponges.