The Comedy Theatre is a 1003-seat theatre in Melbourne's East End Theatre District. It was built in 1928, and was designed in the Spanish style, with a Florentine-style exterior and wrought-iron balconies. It is located at 240 Exhibition Street, and diagonally opposite Her Majesty's Theatre.

The East End Theatre District is a precinct within the Melbourne central business district, and is bounded by Spring, Flinders, Swanston and Lonsdale Streets. The district is home to seven major theatres, including the Princess Theatre, Her Majesty's Theatre and the Regent Theatre. These theatres mostly house commercial productions of musicals, plays and other events, in contrast with the Southbank Arts Precinct over the Yarra River which focuses on publicly funded companies.