Congregation Mikveh Israel, is a Sephardic Orthodox Jewish synagogue located at 44 North Fourth Street in Philadelphia, Pennsylvania, in the United States. The congregation traces its history from 1740. Mikveh Israel is a Spanish and Portuguese congregation that follows the rite of the Amsterdam esnoga. It is the oldest synagogue in Philadelphia, and the longest running in the United States.

Spanish and Portuguese Jews, also called Western Sephardim, Iberian Jews, or Peninsular Jews, are a distinctive sub-group of Sephardic Jews who are largely descended from Jews who lived as New Christians in the Iberian Peninsula during the few centuries following the forced expulsion of unconverted Jews from Spain in 1492 and from Portugal in 1497. They should therefore be distinguished both from the descendants of those expelled in 1492 and from the present-day Jewish communities of Spain and Portugal.