Cowane's Hospital is a 17th-century almshouse in the Old Town of Stirling, Scotland. It was established in 1637 with a bequest of 40,000 merks from the estate of the merchant John Cowane (1570–1633). Subsequently converted for use as a Guildhall the building is considered by Historic Scotland to be "a rare survival of 17th century burgh architecture and one of the finest buildings of its kind in Scotland." It was listed at category A in 1965. The gardens are also seen as a "rare survival" of an institutional garden of the 17th century, and were included in the national Inventory of Gardens and Designed Landscapes in 2012. The hospital is located on St John Street, between the medieval Church of the Holy Rude and the 19th-century Old Town Jail.

Stirling is a city in central Scotland, 26 miles (42 km) northeast of Glasgow and 37 miles (60 km) north-west of Edinburgh. The market town, surrounded by rich farmland, grew up connecting the royal citadel, the medieval old town with its merchants and tradesmen, the Old Bridge and the port. Located on the River Forth, Stirling is the administrative centre for the Stirling council area, and is traditionally the county town of Stirlingshire. Proverbially it is the strategically important "Gateway to the Highlands". It has been said that "Stirling, like a huge brooch clasps Highlands and Lowlands together". Similarly "he who holds Stirling, holds Scotland" is sometimes attributed to Robert the Bruce. Stirling's key position as the lowest bridging point of the River Forth before it broadens towards the Firth of Forth made it a focal point for travel north or south.