The Crowes railway line was a 2 ft 6 in narrow gauge railway located in the Otway Ranges in south-western Victoria, Australia, running from the main line to Port Fairy at Colac to Beech Forest and later to Crowes.

2 ft 6 in gauge railways are narrow gauge railways with track gauge of 2 ft 6 in. This type of rail was promoted especially in the colonies of the British Empire during the second half of the nineteenth century by Thomas Hall and Everard Calthrop.