Cryopreservation or cryoconservation is a process where biological material - cells, tissues, or organs - are frozen to preserve the material for an extended period of time. At low temperatures any cell metabolism which might cause damage to the biological material in question is effectively stopped. Cryopreservation is an effective way to transport biological samples over long distances, store samples for prolonged periods of time, and create a bank of samples for users. Molecules, referred to as cryoprotective agents (CPAs), are added to reduce the osmotic shock and physical stresses cells undergo in the freezing process. Some cryoprotective agents used in research are inspired by plants and animals in nature that have unique cold tolerance to survive harsh winters, including: trees, wood frogs, and tardigrades.
Cryogenically preserved samples being removed from a dewar of liquid nitrogen
Tubes of biological samples being placed in liquid nitrogen
A tank of liquid nitrogen, used to supply a cryogenic freezer (for storing laboratory samples at a temperature of about −150 °C or −238 °F)
Four different ecotypes of Physcomitrella patens stored at the International Moss Stock Center
Liquid nitrogen—LN2—is nitrogen in a liquid state at low temperature. Liquid nitrogen has a boiling point of about −196 °C (−321 °F; 77 K). It is produced industrially by fractional distillation of liquid air. It is a colorless, mobile liquid whose viscosity is about one tenth that of acetone (i.e. roughly one thirtieth that of room temperature water). Liquid nitrogen is widely used as a coolant.
Liquid nitrogen
Students preparing homemade ice cream with a dewar of liquid nitrogen
Filling a liquid nitrogen Dewar from a storage tank