Culham Lock is a lock on the River Thames in England close to Culham, Oxfordshire. It is on a lock cut to the north of the main stream, which approaches the large village of Sutton Courtenay. The lock was built of stone by the Thames Navigation Commission in 1809.

Culham is a village and civil parish in a bend of the River Thames, 1 mile (1.6 km) south of Abingdon in Oxfordshire. The parish includes Culham Science Centre and Europa School UK. The parish is bounded by the Thames to the north, west and south, and by present and former field boundaries to the east. It is low-lying and fairly flat, rising from the Thames floodplain in the south to a north-facing escarpment in the north up to 260 feet (80 m) above sea level. The 2011 Census recorded its population as 453.