Customs House is a heritage-listed customs house at 427 Queen Street, Brisbane CBD, Brisbane, Queensland, Australia. It was designed by Charles H McLay and built from 1886 to 1889 at a cost of £38,346 by John Petrie & Son. It was originally used for the collection of customs duty and was opened in 1889, when Queensland was a British colony, replacing the original Customs House located at Petrie Bight. It was added to the Queensland Heritage Register on 7 February 2005.

Brisbane City is the central suburb and central business district of Brisbane, the state capital of Queensland, Australia. It is colloquially referred to as the "Brisbane CBD" or "the city". It is located on a point on the northern bank of the Brisbane River, historically known as Meanjin, Mianjin or Meeanjin in the local Aboriginal Australian dialect. The triangular shaped area is bounded by the median of the Brisbane River to the east, south and west. The point, known at its tip as Gardens Point, slopes upward to the north-west where the city is bounded by parkland and the inner city suburb of Spring Hill to the north. The CBD is bounded to the north-east by the suburb of Fortitude Valley. To the west the CBD is bounded by Petrie Terrace, which in 2010 was reinstated as a suburb.