Delaware Route 16 (DE 16) is an east-west state highway in Delaware, mainly across northern Sussex County, with a small portion near the Maryland border in extreme southwestern Kent County. It runs from Maryland Route 16 (MD 16) at the Maryland border in Hickman east to the Delaware Bay at Broadkill Beach. The route passes through rural areas along with the towns of Greenwood, Ellendale, and Milton. DE 16 intersects DE 36 and U.S. Route 13 (US 13) in Greenwood, US 113 in Ellendale, DE 30 and DE 5 in the Milton area, and DE 1 between Milton and Broadkill Beach. West of DE 1, the route serves as part of a connection between the Baltimore–Washington Metropolitan Area and the Delaware Beaches. DE 16 was built as a state highway during the 1920s and 1930s. By 1936, the route was designated onto its current alignment.

Sussex County is located in the southern part of the U.S. state of Delaware, on the Delmarva Peninsula. As of the 2020 census, the population was 237,378, making it the state's second most populated county only behind New Castle and ahead of Kent. The county seat is Georgetown.