Diana and Actaeon is a painting by the Italian Renaissance master Titian, finished in 1556–1559, and is considered amongst Titian's greatest works. It portrays the moment in which the hunter Actaeon bursts in where the goddess Diana and her nymphs are bathing. Diana is furious, and will turn Actaeon into a stag, who is then pursued and killed by his own hounds, a scene Titian later painted in his The Death of Actaeon.

Tiziano Vecelli or Vecellio, Latinized as Titianus, hence known in English as Titian, was an Italian (Venetian) Renaissance painter of Lombard origin, considered the most important member of the 16th-century Venetian school. He was born in Pieve di Cadore, near Belluno. During his lifetime he was often called da Cadore, 'from Cadore', taken from his native region.