Diana and Callisto is a painting completed between 1556 and 1559 by the Italian late Renaissance artist Titian. It portrays the moment in which the goddess Diana discovers that her maid Callisto has become pregnant by Jupiter. The painting was jointly purchased by the National Gallery and the Scottish National Gallery for £45 million in March 2012. Along with its companion painting Diana and Actaeon it is displayed on an alternating basis between London and Edinburgh. There is a later version by Titian and his workshop in the Kunsthistorisches Museum in Vienna.

Tiziano Vecelli or Vecellio, Latinized as Titianus, hence known in English as Titian, was an Italian (Venetian) Renaissance painter of Lombard origin, considered the most important member of the 16th-century Venetian school. He was born in Pieve di Cadore, near Belluno. During his lifetime he was often called da Cadore, 'from Cadore', taken from his native region.