Doctor Mid-Nite or Doctor Midnight is the name of multiple fictional superheroes in DC Comics. The figure has been represented in the comics by three different individuals, Charles McNider, Beth Chapel, and Pieter Anton Cross. Dr. Mid-Nite was originally created by writer Charles Reizenstein and artist Stanley Josephs Aschmeier in 1941. The hero, represented first by Charles McNider, appeared for the first time in All-American Comics #25. He continued in All-American Comics until issue #102.

Dr. Elizabeth "Beth" Chapel is a fictional superheroine in American comic books published by DC Comics. She is the second character to adopt the Doctor Mid-Nite codename and is the successor of the original, Charles McNider. The character is later succeeded by Pieter Cross after her death at the hands of Eclipso. However, following the events of Doomday Clock, the character is resurrected. Similar to her predecessor, the character possesses the same abilities as Charles and primarily functions as one of the superhero community's renowned physicians. She serves as the official physician for both Infinity, Inc. and the Justice Society of America.