The 2007 Tour de France was affected by a series of scandals and speculations related to doping. By the end of the Tour, two cyclists were dismissed for failing tests and the wearer of the yellow jersey was voluntarily retired by his team for lying about his whereabouts and missing doping tests. A fourth rider was confirmed to having used doping while in a training session prior to the 2007 Tour and a fifth rider failed tests late in the race, with his result being officially announced just after the end of the Tour. During the competition, two teams were asked to withdraw after at least one member was found to have doped.

Johan Bruyneel is a Belgian former professional road bicycle racer and a former directeur sportif for UCI ProTour team RadioShack–Nissan, and U.S. Postal Service, a US-based UCI ProTour cycling team. On 25 October 2018, the World Anti Doping Agency imposed a lifetime ban on Bruyneel for his role in a doping scandal that also saw Lance Armstrong stripped of his seven Tour de France titles.