The Eberswalde–Frankfurt (Oder) railway is a single-track line in the districts of Barnim and Märkisch-Oderland and the town of Frankfurt (Oder), in the German state of Brandenburg. The section from Eberswalde to Werbig junction is now a branch line, the adjoining section to the south to Frankfurt (Oder) is classified as a main line. The line is about 86 kilometres long and is served by line RB60 of the Niederbarnimer Eisenbahn (NEB).

Eberswalde Hauptbahnhof is historically the most important and now the only remaining station in the city of Eberswalde in the German state of Brandenburg. It was opened in the summer of 1842 outside the then city limits on the Berlin–Szczecin railway. The city fathers of Eberswalde did not want a modern railway in their city, so the station was built three kilometres west of the city centre in a wooded area where the Westend district is today.