Electroporation
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Electroporation, also known as electropermeabilization, is a microbiological and biotechnological technique in which an electric field is applied to cells to briefly increase the permeability of the c
Cuvettes for in-vitro electroporation. These are plastic with aluminium electrodes and a blue lid. They hold a maximum of 400 μL.
Electrogenetransfer
Hybridoma technology
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Hybridoma technology is a method for producing large quantities of monoclonal antibodies by fusing antibody producing B cells with myeloma cells. This creates hybrid cells, hybridomas, that produce th
Electroporation
…on the specific cell type and cargo characteristics. Electroporation is also employed to induce cell fusion. A prominent application of cell fusion is hybridoma technology, where antibody-producing B lymphocytes are fused with immortal myeloma cell lines to produce monoclonal antibodies. Electroporation is widely utilized…
Hybridoma cells grown in tissue culture. The image shows a single clone of cells each of which is producing large amounts of a specific monoclonal antibody which the cells secrete and which can be readily purified from the culture media.