Elizabeth Seymour was a younger daughter of Sir John Seymour of Wulfhall, Wiltshire and Margery Wentworth. Elizabeth and her sister Jane served in the household of Anne Boleyn, the second wife of Henry VIII. The Seymours rose to prominence after the king's attention turned to Jane. In May 1536, Anne Boleyn was accused of treason and adultery, and subsequently executed. On 30 May 1536, eleven days after Anne's execution, Henry VIII and Jane were married. Elizabeth was not included in her sister's household during her brief reign, although she would serve two of Henry VIII's later wives, Anne of Cleves and Catherine Howard. Jane died 24 October 1537, twelve days after giving birth to a healthy son, Edward VI.

Sir John Seymour, Knight banneret was an English soldier and a courtier who served both Henry VII and Henry VIII. Born into a prominent gentry family, he is best known as the father of Henry VIII's third wife, Jane Seymour, and hence grandfather of king Edward VI of England.