Estero is an incorporated village in Lee County, Florida, United States, located directly beside the first aquatic nature preserve established in Florida: The Estero Bay Aquatic Preserve, otherwise referred to as Estero Bay Preserve State Park which is within Estero Bay, Florida. At the time of the 2010 census, Estero was an unincorporated community and census-designated place, but incorporated as a village on the last calendar date of 2014. It is part of the Cape Coral-Fort Myers, Florida Metropolitan Statistical Area. As of the 2020 census, the population was 36,939.

Mound Key Archaeological State Park is a Florida State Park, located in Estero Bay, near the mouth of the Estero River. One hundred and thirteen of the island's one hundred and twenty-five acres are managed by the park system. It is a complex of mounds and accumulated shell, fish bone, and pottery middens that rises more than 30 feet above the waters of the bay.