Ezio Auditore da Firenze is a character in the video game series Assassin's Creed, an Italian Master Assassin who serves as the protagonist of the series' games set during the Italian Renaissance. His life and career as an Assassin are chronicled in Assassin's Creed II, II: Discovery, Brotherhood (2010), and Revelations (2011), as well as the short films Assassin's Creed: Lineage (2009), Ascendance (2010), and Embers (2011) and various spin-off media of the franchise. In 2016, all three major games featuring Ezio, as well as Lineage and Embers, were re-released as an enhanced bundle titled Assassin's Creed: The Ezio Collection. Throughout most of his appearances, the character has been voiced by American actor Roger Craig Smith, while Canadian actor Devon Bostick portrayed him in live-action in Lineage.

Assassin's Creed is a historical action-adventure video game series and media franchise published by Ubisoft and developed mainly by its studio Ubisoft Montreal using the game engine Anvil and its more advanced derivatives. Created by Patrice Désilets, Jade Raymond, and Corey May, the Assassin's Creed video game series depicts a fictional millennia-old struggle between the Order of Assassins, who fight for peace and free will, and the Knights Templar, who desire peace through order and control. The series features historical fiction, science fiction, and fictional characters intertwined with real-world historical events and historical figures. In most games, players control a historical Assassin while also playing as an Assassin Initiate or someone caught in the Assassin–Templar conflict in the present-day framing story. Considered a spiritual successor to the Prince of Persia series, Assassin's Creed took inspiration from the novel Alamut by the Slovenian writer Vladimir Bartol, based on the historical Hashashin sect of the medieval Middle East.