FC Spartak Trnava is a professional football club based in Trnava, Slovakia. The club competes in Niké Liga, the top division in the Slovak football league system, having participated in more seasons than any other club.

Trnava is a city in western Slovakia, 47 km (29 mi) to the northeast of Bratislava, on the Trnávka river. It is the capital of the Trnava Region and the Trnava District. It is the seat of a Roman Catholic archbishopric. The city has a historic center. Because of the many churches within its city walls, Trnava has often been called "Little Rome", or more recently, the "Slovak Rome".