John Twynyho of Cirencester, Bristol and Lechlade, all in Gloucestershire, was a lawyer and wealthy wool merchant who served as Recorder of Bristol, as a Member of Parliament for Bristol in Gloucestershire in 1472-5 and in 1484 and for the prestigious county seat Gloucestershire in 1476. In 1478 he was Attorney General to Lord Edward (the future King Edward V, eldest son and heir of King Edward IV.