The First Unitarian Church of Rochester is located at 220 Winton Road South in Rochester, New York, U.S. The congregation is one of the largest in its denomination, the Unitarian Universalist Association. The non-creedal church conducts programs in the areas of spirituality, social concerns, music, and arts. This church is one of two Unitarian Universalist congregations in Monroe County, the other being First Universalist Church of Rochester.

Unitarian Universalism is a liberal religious movement characterized by a "free and responsible search for truth and meaning". Unitarian Universalists assert no creed, but instead are unified by their shared search for spiritual growth. Unitarian Universalists do not have an official, unified corpus of sacred texts but rather draw inspiration and guidance from the six sources: personal experience, prophetic utterances, world religions, Jewish and Christian teachings, humanist teachings, and spiritual teachings. Unitarian Universalist congregations include many atheists, agnostics, deists, and theists; there are churches, fellowships, congregations, and societies around the world.