State Road 16 (SR 16) runs from northwest to southeast between Raiford and St. Augustine. It passes through the towns of Starke and Green Cove Springs in addition to providing access to Camp Blanding. Major roads and/or highways that SR 16 crosses include: US 301, SR 21, US 17, SR 13, Interstate 95 and US 1.

Several special routes of U.S. Route 1 (US 1) exist, from Florida to Maine. In order from south to north, separated by type, these special routes are as follows.