State Road 29 is a state highway that runs north–south through Southwest Florida. It begins in Carnstown and runs north to a point just south of Palmdale. A rural road, it runs mostly through uninhabited farmland in its northern half, and along wetlands in its southern half. The route previously continued south of Carnestown to Everglades City and Chokoloskee, which has since become County Road 29.

U.S. Highway 41 (US 41) in the state of Florida is a north–south United States Highway. It runs 479 miles (771 km) from Miami in South Florida northwest to the Georgia state line north of the Lake City area. Within the state, US 41 is paralleled by Interstate 75 (I-75) all the way from Miami to Georgia, and I-75 has largely supplanted US 41 as a major highway.