State Road 29 is a state highway that runs north–south through Southwest Florida. It begins in Carnstown and runs north to a point just south of Palmdale. A rural road, it runs mostly through uninhabited farmland in its northern half, and along wetlands in its southern half. The route previously continued south of Carnestown to Everglades City and Chokoloskee, which has since become County Road 29.

Everglades City is a city in Collier County, Florida, United States, of which it was once the county seat. The City of Everglades City is part of the Naples–Marco Island Metropolitan Statistical Area, which is included in the Cape Coral-Fort Myers-Naples Combined Statistical Area. The Gulf Coast Visitor Center for Everglades National Park is located in Everglades City. As of the 2020 US census, the population was 352, down from 400 in the 2010 US census.