Fort Assiniboine is a hamlet in northwest Alberta, Canada, within Woodlands County. It is located along the north shore of the Athabasca River at the junction of Highway 33 and Highway 661. It is approximately 39 kilometres (24 mi) northwest of Barrhead, 62 kilometres (39 mi) southeast of Swan Hills and 91 kilometres (57 mi) northeast of Whitecourt.

Woodlands County is a municipal district in north-central Alberta, Canada. Located in Census Division No. 13, its municipal office is located outside but adjacent to the Town of Whitecourt. A second municipal office is located in the Hamlet of Fort Assiniboine.