Gene knockout
Videos
Gene knockouts are a widely used genetic engineering technique that involves the targeted removal or inactivation of a specific gene within an organism's genome. This can be done through a variety of
A laboratory mouse in which a gene affecting hair growth has been knocked out (left), is shown next to a normal lab mouse.
Wild-type Physcomitrella and knockout mosses: Deviating phenotypes induced in gene-disruption library transformants. Physcomitrella wild-type and transformed plants were grown on minimal Knop medium to induce differentiation and development of gametophores. For each plant, an overview (upper row; scale bar corresponds to 1 mm) and a close-up (bottom row; scale bar equals 0.5 mm) are shown. A: Haploid wild-type moss plant completely covered with leafy gametophores and close-up of wild-type leaf. B–E: Different mutants.
Frameshift mutation resulting from a single base pair deletion, causing altered amino acid sequence and premature stop codon
A knockout mouse (left) that is a model of obesity, compared with a normal mouse
Gene targeting
Videos
Gene targeting is a biotechnological tool used to change the DNA sequence of an organism. It is based on the natural DNA-repair mechanism of homology directed repair, including homologous recombinatio
Gene knockout
…Gene knockouts (also known as gene deletion or gene inactivation) are a widely used genetic engineering technique that involves the targeted removal or inactivation of a specific gene within an organism's genome. This can be done through a variety of methods, including homologous recombination…
A chimeric mouse gene targeted for the agouti coat color gene, with its offspring