The Genocide of Serbs in the Independent State of Croatia was the systematic persecution of Serbs committed during World War II by the fascist Ustaše regime in the Nazi German puppet state known as the Independent State of Croatia between 1941 and 1945. It was carried out through executions in death camps, as well as through mass murder, ethnic cleansing, deportations, forced conversions, and war rape. This genocide was simultaneously carried out with the Holocaust in the NDH as well as the genocide of Roma, by combining Nazi racial policies with the ultimate goal of creating an ethnically pure Greater Croatia.

The Ustaše, also known by anglicised versions Ustasha or Ustashe, was a Croatian, fascist and ultranationalist organization active, as one organization, between 1929 and 1945, formally known as the Ustaša – Croatian Revolutionary Movement. Since the inception and before the Second World War, its members engaged in a series of terrorist activities against the Kingdom of Yugoslavia, including collaborating with IMRO to assassinate King Alexander I of Yugoslavia in 1934. After the invasion of Yugoslavia by Axis forces in April 1941, the Ustaše were handed power by the occupying forces and formed the Independent state of Croatia, an Axis puppet state, and immediately went on to perpetrate the Holocaust and genocide against its Jewish, Serb and Roma populations. During the World War II in Yugoslavia, together with German and Italian occupying forces, Ustaše are responsible for killing of hundreds of thousands of Serbs, Jews, Roma, as well as Bosniak Muslim and Croat political dissidents.