Glade Spring is a town in Washington County, Virginia, United States. The population was 1,456 at the 2010 census. It is part of the Kingsport–Bristol (TN)–Bristol (VA) Metropolitan Statistical Area, which is a component of the Johnson City–Kingsport–Bristol, TN-VA Combined Statistical Area – commonly known as the "Tri-Cities" region. I-81 runs through Glade Spring. It is located at exit 29.

Washington County is a county located in the Commonwealth of Virginia. As of the 2020 census, the population was 53,935. Its county seat is Abingdon.