The Glasgow, Paisley, Kilmarnock and Ayr Railway (GPK&AR) was a railway in Scotland that provided train services between Glasgow, Kilmarnock and Ayr. It opened its first line, between Glasgow and Ayr, in stages from 1839 to 1840. The section between Glasgow and Paisley was made jointly with the Glasgow, Paisley and Greenock Railway. Later it built a line from Dalry via Kilmarnock to Cumnock, linking there with the Glasgow, Dumfries and Carlisle Railway, and together forming a through route from Glasgow to Carlisle. The two companies merged to form the Glasgow and South Western Railway.

Ayr is a town situated on the southwest coast of Scotland. A former royal burgh, today it is the administrative centre of the South Ayrshire Council, and the historic county town of Ayrshire. With a population of 46,982, Ayr is the 15th largest settlement in Scotland and second largest town in Ayrshire by population. The town is contiguous with the smaller town of Prestwick to the north. Ayr submitted unsuccessful bids for city status in 2000 and 2002, and as part of the wider South Ayrshire area in 2022.