The Gloucestershire Wildlife Trust is the Gloucestershire local partner in a conservation network of 46 Wildlife Trusts. The Wildlife Trusts are local charities with the specific aim of protecting the United Kingdom's natural heritage. The Gloucestershire Wildlife Trust is managed by a board of trustees elected from its membership who provide overall direction for the development of the trust and there are advisory committees. The work of the trust is carried out through staff and volunteers.

The Wildlife Trusts, the trading name of the Royal Society of Wildlife Trusts, is an organisation made up of 46 local Wildlife Trusts in the United Kingdom, the Isle of Man and Alderney. The Wildlife Trusts, between them, look after more than 2,300 nature reserves, covering around 98,500 hectares. As of 2020, the Trusts have a combined membership of over 870,000 members.