"Go Down Moses" is an African American spiritual that describes the Hebrew exodus, specifically drawing from the Book of Exodus 5:1: "And the LORD spoke unto Moses, Go unto Pharaoh, and say unto him, Thus saith the LORD, Let my people go, that they may serve me", where God commands Moses to demand the release of the Israelites from bondage in Egypt. As is common in spirituals, the song discusses freedom, referring both to the freedom of the Israelites, and that of runaway enslaved people. As a result of these messages, this song was outlawed by many enslavers.

Spirituals is a genre of Christian music that is associated with African Americans, which merged varied African cultural influences with the experiences of being held in bondage in slavery, at first during the transatlantic slave trade and for centuries afterwards, through the domestic slave trade. Spirituals encompass the "sing songs", work songs, and plantation songs that evolved into the blues and gospel songs in church. In the nineteenth century, the word "spirituals" referred to all these subcategories of folk songs. While they were often rooted in biblical stories, they also described the extreme hardships endured by African Americans who were enslaved from the 17th century until the 1860s, the emancipation altering mainly the nature of slavery for many. Many new derivative music genres such as the blues emerged from the spirituals songcraft.