The government of Florida is established and operated according to the Constitution of Florida and is composed of three branches of government: the executive branch consisting of the governor of Florida and the other elected and appointed constitutional officers; the legislative branch, the Florida Legislature, consisting of the Senate and House; and the judicial branch consisting of the Supreme Court of Florida and lower courts. The state also allows direct participation of the electorate by initiative, referendum, and ratification.

The Florida Senate is the upper house of the Florida Legislature, the state legislature of the U.S. state of Florida, the Florida House of Representatives being the lower house. Article III, Section 1 of the Constitution of Florida, adopted in 1968, defines the role of the Legislature and how it is to be constituted. The Senate is composed of 40 members, each elected from a single-member district with a population of approximately 540,000 residents. Legislative districts are drawn on the basis of population figures, provided by the federal decennial census. Senators' terms begin immediately upon their election. The Senate Chamber is located in the State Capitol building.