The Great Fire of 1901 was a conflagration that occurred in Jacksonville, Florida, on May 3, 1901. It was one of the worst disasters in Florida history and the third largest urban fire in the U.S., next to the Great Chicago Fire, and the 1906 San Francisco fire.

A conflagration is a large fire. Conflagrations often damage human life, animal life, health, and/or property. A conflagration can begin accidentally or be intentionally created (arson). A very large fire can produce a firestorm, in which the central column of rising heated air induces strong inward winds, which supply oxygen to the fire. Conflagrations can cause casualties including deaths or injuries from burns, trauma due to collapse of structures and attempts to escape, and smoke inhalation.