The Great fire of Brisbane was a major conflagration that swept through the central parts of Brisbane in the Colony of Queensland on 1 December 1864. For two and a half hours the fire burned out of control in large parts of Brisbane's central business district with entire blocks being destroyed, mainly in Queen, Albert, George, and Elizabeth Streets. It consumed 50 houses, 2 banks, 3 hotels, 4 draperies, and many other businesses as well as a "considerable amount of small houses". Considering the extent of the fire, casualties were very few; there was no loss of life, and four people were taken to hospital with injuries.

A conflagration is a large fire. Conflagrations often damage human life, animal life, health, and/or property. A conflagration can begin accidentally or be intentionally created (arson). A very large fire can produce a firestorm, in which the central column of rising heated air induces strong inward winds, which supply oxygen to the fire. Conflagrations can cause casualties including deaths or injuries from burns, trauma due to collapse of structures and attempts to escape, and smoke inhalation.