The Guajira Peninsula is a peninsula in northern Colombia and northwestern Venezuela in the Caribbean. It is the northernmost peninsula in South America and has an area of 25,000 km2 (9,700 sq mi) extending from the Manaure Bay (Colombia) to the Calabozo Ensenada in the Gulf of Venezuela (Venezuela), and from the Caribbean to the Serranía del Perijá mountains range.

The Spanish colonization of the Americas began in 1493 on the Caribbean island of Hispaniola after the initial 1492 voyage of Genoese mariner Christopher Columbus under license from Queen Isabella I of Castile. These overseas territories of the Spanish Empire were under the jurisdiction of Crown of Castile until the last territory was lost in 1898. Spaniards saw the dense populations of indigenous peoples as an important economic resource and the territory claimed as potentially producing great wealth for individual Spaniards and the crown. Religion played an important role in the Spanish conquest and incorporation of indigenous peoples, bringing them into the Catholic Church peacefully or by force. The crown created civil and religious structures to administer the vast territory. Spanish men and women settled in greatest numbers where there were dense indigenous populations and the existence of valuable resources for extraction.