The Guelph Gryphons football team represents the University of Guelph in Guelph, Ontario in the sport of Canadian football in the Ontario University Athletics conference of U Sports. The Guelph Gryphons football team has been in continuous operation since 1950. The team has won one Vanier Cup national championship in 1984, which is also their only appearance in the title game. The Gryphons are the only program with a perfect record in Vanier Cup games. The team has also won four Yates Cup conference championships, in 1984, 1992, 1996 and 2015.

The Vanier Cup is a post season college football championship game, used to determine the national champion in U Sports football. The game is the final for the winners of the Uteck Bowl and the Mitchell Bowl. In turn, the participating teams are determined by the winners of 4 bowl games: the Loney Bowl (AUS), Hardy Cup, Dunsmore Cup (RSEQ), and Yates Cup (OUA).