Guyana National Museum is a museum in Georgetown, Demerara-Mahaica, Guyana. It was established on 13 February 1868. The idea of starting a museum was conceived by members of the Royal Agricultural and Commercial Society (RACS) of British Guiana. When RACS was established in 1844, one of its aims was to construct a Museum to house local minerals, soils, timbers, fruits, seeds, gums, resins, dyes and drugs, as well as the flora and fauna of the country. British explorer Robert Schomburgk, the German botanist Carl Ferdinand Appun, Mr Bratt, and W.H. Campbell presented gifts to the RACS in order to start a Museum Collection. A fire in 1864 destroyed the donated collections.

Georgetown is the capital and largest city of Guyana. It is situated in Demerara-Mahaica, region 4, on the Atlantic Ocean coast, at the mouth of the Demerara River. It is nicknamed the "Garden City of the Caribbean." It is the retail, administrative, and financial services centre of the country, and the city accounts for a large portion of Guyana's GDP. The city recorded a population of 118,363 in the 2012 census.