Haplogroup L3 is a human mitochondrial DNA haplogroup. The clade has played a pivotal role in the early dispersal of anatomically modern humans.
Proposed migration route depicting the origin of L3 in Africa and its dispersal both out of and within the continent, with two possible models (as depicted by Vai et al.) (a) Haplogroup N differentiates from L3 in Africa, with a subsequent spread out of Africa, and differentiation of haplogroup M from L3 outside Africa. (b) Haplogroups M and N diverge from L3 outside Africa after the expansion of L3 from the continent; later migrations during the Upper Paleolithic and Neolithic led some lineages back to North Africa. (Haplogroups are indicated in black circles in their probable area of origin.)